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Addgene inc a chenn16
A Chenn16, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+pcag+cre+ires2+gfp/pCAG-Cre-IRES2-GFP+(Plasmid+%2326646)/us12570703-293-24-26
Average 93 stars, based on 76 article reviews
a chenn16 - by Bioz Stars, 2026-09
93/100 stars

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Selection:

Article Title: A Universal Approach to Correct Various HBB Gene Mutations in Human Stem Cells for Gene Therapy of Beta‐Thalassemia and Sickle Cell Disease
Article Snippet: .. To excise the loxP‐flanked PGK‐puromycin selection cassette, human iPSCs were transfected with the plasmid pCAG‐Cre‐IRES2‐GFP (Addgene, #26646) as done previously . .. After 3 days of transfection, the GFP positive cells were selected by fluorescence‐activated cell sorting on FACSAria II (BD Biosciences, San Jose, CA).

Transfection:

Article Title: A Universal Approach to Correct Various HBB Gene Mutations in Human Stem Cells for Gene Therapy of Beta‐Thalassemia and Sickle Cell Disease
Article Snippet: .. To excise the loxP‐flanked PGK‐puromycin selection cassette, human iPSCs were transfected with the plasmid pCAG‐Cre‐IRES2‐GFP (Addgene, #26646) as done previously . .. After 3 days of transfection, the GFP positive cells were selected by fluorescence‐activated cell sorting on FACSAria II (BD Biosciences, San Jose, CA).

Plasmid Preparation:

Article Title: A Universal Approach to Correct Various HBB Gene Mutations in Human Stem Cells for Gene Therapy of Beta‐Thalassemia and Sickle Cell Disease
Article Snippet: .. To excise the loxP‐flanked PGK‐puromycin selection cassette, human iPSCs were transfected with the plasmid pCAG‐Cre‐IRES2‐GFP (Addgene, #26646) as done previously . .. After 3 days of transfection, the GFP positive cells were selected by fluorescence‐activated cell sorting on FACSAria II (BD Biosciences, San Jose, CA).

Article Title: Lipidoids for nucleic acid transfection and use thereof
Article Snippet: .. Biodistribution of New mRNA-LNPs In Vivo The gene encoding the CRE recombinase protein was amplified from the plasmid pCAG-Cre-IRES2-GFP (Addgene, catalog number 26646, donated by Anjen Chenn) using the primers: (5′-TAATACGACTCACTATAGAATTTACT-′3 (SEQ ID NO. 9); 5′-CTAATCGCCATCTTCCAGCA-3′ (SEQ ID NO. 10)). ..

Article Title: Lipidoids for nucleic acid transfection and use thereof
Article Snippet: .. Effectivity of Nucleoside-Modified mRNA-LNPs In Vivo The gene encoding the CRE recombinase protein was amplified from the plasmid pCAG-Cre-IRES2-GFP (Addgene, catalog number 26646, donated by Anjen Chenn) using the primers: (5′-TAATACGACTCACTATAGAATTTACT-′3 (SEQ ID NO. 9); 5′-CTAATCGCCATCTTCCAGCA-3′ (SEQ ID NO. 10)). .. Messenger RNA (mRNA) was prepared in vitro analogously to Example 25, with the exception that CTP was 100% exchanged for 5-Methyl-CTP (NU-1138L, Biogen Praha s.r.o.) and UTP was 100% exchanged for N1-Methylpseudo-UTP (NU-890L, Biogen Praha s.r.o.), and packaged into LNP as follows: A 300 μl sample of transfection reagent A28, and 120 μg of mRNA in 300 μl of 10 mM citrate buffer (pH 3.0) were assembled into LNP using a microfluidic device analogously to Example 25.

In Vivo:

Article Title: Lipidoids for nucleic acid transfection and use thereof
Article Snippet: .. Biodistribution of New mRNA-LNPs In Vivo The gene encoding the CRE recombinase protein was amplified from the plasmid pCAG-Cre-IRES2-GFP (Addgene, catalog number 26646, donated by Anjen Chenn) using the primers: (5′-TAATACGACTCACTATAGAATTTACT-′3 (SEQ ID NO. 9); 5′-CTAATCGCCATCTTCCAGCA-3′ (SEQ ID NO. 10)). ..

Article Title: Lipidoids for nucleic acid transfection and use thereof
Article Snippet: .. Effectivity of Nucleoside-Modified mRNA-LNPs In Vivo The gene encoding the CRE recombinase protein was amplified from the plasmid pCAG-Cre-IRES2-GFP (Addgene, catalog number 26646, donated by Anjen Chenn) using the primers: (5′-TAATACGACTCACTATAGAATTTACT-′3 (SEQ ID NO. 9); 5′-CTAATCGCCATCTTCCAGCA-3′ (SEQ ID NO. 10)). .. Messenger RNA (mRNA) was prepared in vitro analogously to Example 25, with the exception that CTP was 100% exchanged for 5-Methyl-CTP (NU-1138L, Biogen Praha s.r.o.) and UTP was 100% exchanged for N1-Methylpseudo-UTP (NU-890L, Biogen Praha s.r.o.), and packaged into LNP as follows: A 300 μl sample of transfection reagent A28, and 120 μg of mRNA in 300 μl of 10 mM citrate buffer (pH 3.0) were assembled into LNP using a microfluidic device analogously to Example 25.

Amplification:

Article Title: Lipidoids for nucleic acid transfection and use thereof
Article Snippet: .. Biodistribution of New mRNA-LNPs In Vivo The gene encoding the CRE recombinase protein was amplified from the plasmid pCAG-Cre-IRES2-GFP (Addgene, catalog number 26646, donated by Anjen Chenn) using the primers: (5′-TAATACGACTCACTATAGAATTTACT-′3 (SEQ ID NO. 9); 5′-CTAATCGCCATCTTCCAGCA-3′ (SEQ ID NO. 10)). ..

Article Title: Lipidoids for nucleic acid transfection and use thereof
Article Snippet: .. Effectivity of Nucleoside-Modified mRNA-LNPs In Vivo The gene encoding the CRE recombinase protein was amplified from the plasmid pCAG-Cre-IRES2-GFP (Addgene, catalog number 26646, donated by Anjen Chenn) using the primers: (5′-TAATACGACTCACTATAGAATTTACT-′3 (SEQ ID NO. 9); 5′-CTAATCGCCATCTTCCAGCA-3′ (SEQ ID NO. 10)). .. Messenger RNA (mRNA) was prepared in vitro analogously to Example 25, with the exception that CTP was 100% exchanged for 5-Methyl-CTP (NU-1138L, Biogen Praha s.r.o.) and UTP was 100% exchanged for N1-Methylpseudo-UTP (NU-890L, Biogen Praha s.r.o.), and packaged into LNP as follows: A 300 μl sample of transfection reagent A28, and 120 μg of mRNA in 300 μl of 10 mM citrate buffer (pH 3.0) were assembled into LNP using a microfluidic device analogously to Example 25.



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